Laboratory Reference
Research Protocols
Reconstitution, storage, and common laboratory study designs. Educational only.
1. Reconstitution (Lab Standard)
Most Physis peptides ship as lyophilized (freeze-dried) powder for maximum stability. In the lab they are typically reconstituted with bacteriostatic water (BAC water) or sterile water before use in experiments.
- Allow the vial to reach room temperature before opening (reduces moisture condensation).
- Wipe the rubber stopper with alcohol.
- Draw the desired volume of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the side of the vial wall — do not blast the powder with the stream.
- Gently swirl or roll the vial until the powder fully dissolves. Do not shake vigorously.
- Label the vial with concentration, date, and researcher initials.
Example concentration math
If a 10 mg vial is reconstituted with 2 mL of water → concentration = 5 mg/mL.
To draw 500 mcg (0.5 mg) you would draw 0.1 mL (10 units on a U-100 insulin syringe).
2. Storage Guidelines
Lyophilized (unreconstituted)
- Store at −20 °C (freezer) for long-term
- Short-term: 2–8 °C (refrigerator) is acceptable
- Protect from light and moisture
- Shelf life often 12–24+ months when frozen
After reconstitution
- Refrigerate at 2–8 °C
- Typical lab use window: 14–30 days
- Do not freeze reconstituted peptides repeatedly
- Discard if cloudy or discolored
3. Common Research Protocol Ranges
Ranges below are frequently cited in peer-reviewed and preclinical literature. Always design protocols under institutional review / lab SOPs.
Healing & Recovery (BPC-157 / TB-500 / Wolverine / GLOW)
BPC-157
Research range often 200–500 mcg once or twice daily. Study lengths 2–6 weeks common in soft-tissue models.
TB-500
Loading: 2–5 mg twice weekly for 4–6 weeks; then maintenance 2–5 mg every 1–2 weeks.
Wolverine blend
Often run together so both regenerative pathways are available in the same research window.
GLOW
Adds GHK-Cu for skin/collagen-focused arms of recovery studies.
Metabolic (Retatrutide / Tirzepatide / 5-Amino-1MQ / MOTS-c)
Retatrutide
Clinical research has used weekly escalating doses (starting ~1–2 mg, titrating higher). Always protocol-dependent.
Tirzepatide
Common schedule: once weekly, starting low (2.5 mg) and escalating every 4 weeks.
MOTS-c
Research often uses 5–15 mg several times per week for metabolic and exercise-mimetic studies.
5-Amino-1MQ
Oral research compound; literature frequently cites ~50–150 mg/day in animal/metabolic designs.
Longevity, Cognitive & Sleep
Epitalon
Cyclic designs: 5–10 mg/day for 10–20 days, repeated 1–2× per year in longevity literature.
Selank
Often 250–500 mcg/day for cognitive/anxiety models over 2–4 weeks.
DSIP
Sleep studies historically used ~100–300 mcg, often evening in experimental designs.
GHK-Cu
1–2 mg/day systemic research; also used topically in dermal studies.
Other Research Protocols
PT-141 (Bremelanotide)
Research doses often 0.5–2 mg; studied extensively in sexual-function models.
KPV
200–500 mcg/day common in gut and anti-inflammatory research.
4. Study Duration Notes
Short studies (1–4 weeks): Acute recovery, sleep, or single-endpoint cognitive models.
Medium studies (4–12 weeks): Metabolic, body-composition, and soft-tissue repair research.
Cyclic / long-term: Longevity peptides (e.g. Epitalon) often appear in pulsed protocols rather than continuous year-round use.